Intradermal (0.1 ml).
An allergic test is placed on the inner surface of the middle third of the forearm. The skin at the injection site of the allergen is pretreated with 70% ethyl alcohol.
Opening of ampoules and the procedure for administering the drug are carried out with strict adherence to aseptic and antiseptic rules. An opened ampoule with an allergen, preserved in accordance with the rules of asepsis, can be used within 2 hours.
0.1 ml of the drug (1 dose) is syringed with a capacity of 1 ml with a thin needle and injected strictly intradermally, so that a small papule with a diameter of 3-5 mm is formed at the site of administration.
Incorporation of reaction results
It is carried out 24-48 hours after the injection of the allergen by examination and palpation of the skin. In some cases, the allergic reaction becomes positive to 72 hours.
With a positive reaction A reddish or pale, painful swelling of an elongated or oval form appears on the site of the allergen injection. Edema can be well contoured with a clear elevation above normal skin.
With a mild reaction Edema is only recognized by palpation (compare with a similar area of skin on the other hand).
Hyperemia of the skin in the absence of edema is taken as negative result.
When taking into account the reaction, the size of the edema in centimeters (length and width), the degree of soreness after 24 and 48 hours are noted. If the result is negative, the reaction should also be taken into account after 72 hours.
The presence of pronounced skin edema at the site of the allergen is considered a positive allergic reaction. Absence of pain and hyperemia in the presence of edema does not exclude a positive evaluation of the sample.
The reaction, which appeared and disappeared earlier than six hours after the administration of the allergen, is considered nonspecific.
Evaluation of reaction results:
- Slightly positive - mild edema less than 2 cm in diameter;
- positive - edema of 2 to 6 cm in diameter;
- sharply positive - edema over 6 cm, sometimes accompanied by lymphadenitis and a general reaction of the body.
The introduction of the brucellosis allergen is recorded in the prescribed registration forms with the name of the preparation, the date of formulation, the dose, the name of the manufacturer, the serial number, the reaction to the administration.
Precautions for use
Given the possibility of developing an anaphylactic shock in certain highly sensitive individuals, the subject should be under medical supervision for at least 30 minutes.Locations of the intradermal test should be provided with anti-shock therapy.