Method of application - Skin - scarification (by 0,05 ml).
An allergic test is placed on the outer surface of the middle third of the left shoulder. The skin at the site of application of the allergen is pretreated with 70% ethyl alcohol.
Opening of ampoules and the procedure for administering the drug are carried out with strict adherence to aseptic and antiseptic rules. An opened ampoule with an allergen, preserved in accordance with the rules of asepsis, can be used within 2 hours.
Before using the ampoule with the drug should be shaken several times until the liquid containing it becomes evenly turbid.One drop of the drug 0.05 ml (1 dose) with a 1 ml syringe with a thin needle is applied to dried skin after treatment. The osprivivivatelnym pen through the applied drop make on the skin two parallel incisions, the distance between which is 2-3 mm, the length is 8-10 mm. Then the drop is rubbed into the notches by the flat side of the ospivyvatelnogo pen for 30 seconds. The incisions should not be excessively deep or, on the contrary, very shallow. After their application, blood should appear in the form of dewdrops.
Incorporation of reaction results
After 24 hours, redness and swelling around the notches appear, reaching a maximum after 48-72 hours. After that, it gradually fades, disappearing completely by 7-10-12 days. At the place of incisions, by this time, only trace marks remain. In rare cases, vesicles appear on the line of incisions, disappearing after 2-3 days.
The results are monitored by the doctor after 48 hours for a skin reaction in the form of hyperemia and edema (infiltration) at the site of application of the drug. To assess its intensity, determine the reactive area of the skin, along the border of hyperemia, measure it across the incisions made.
The reaction is considered positive in the presence of hyperemia and infiltration of the skin not less than 0.5 cm.
Negative reaction consider the absence of cutaneous manifestations, the development of hyperemia without infiltration or the presence of hyperemia and infiltration less than 0.5 cm.
The administration of the tularemia allergen is recorded in the prescribed registration forms with the name of the preparation, the date of administration, the dose, the name of the manufacturer, the serial number, the reaction to the administration.
Precautions for use
Given the possibility of developing an anaphylactic shock in certain highly sensitive individuals, the subject should be under medical supervision for at least 30 minutes. Sample sites should be provided with anti-shock therapy.