Vaccination is carried out once dermally or subcutaneously. One dose for skin administration is 2 drops (0.1 ml) and contains 1x1010 mc, with a subcutaneous injection of 0.5 ml and contains 4x108 m. Revaccination is carried out according to the indications after 10-12 months dermally, using a half dose, which is 1 drop (0.05 ml) and contains 5 x 109 m.
In order to identify contraindications, the doctor (paramedic) on the day of vaccination conducts a survey and examination of the vaccine with mandatory thermometry. At a temperature above 37 ° C, the graft is delayed. If necessary, conduct the necessary laboratory examination.
Before each vaccination, the vaccinee is required to determine the presence of specific immunity with the help of one of the serological or skin-allergic reactions. Inoculations are subject to negative reaction.
Vaccinations should be carried out no later than 3-4 weeks before the start of work associated with the risk of infection.
The vaccination is recorded in the prescribed registration forms with the name of the drug, the date of vaccination, the dose, the name of the manufacturer of the drug, the serial number, the reaction to the vaccination.
Skin Vaccination
The drug is dissolved with sodium chloride solution for injection 0.9%, which is introduced into the ampoule with a sterile syringe with a needle at a rate of 0.1 ml per one inoculation dose. The ampoule is shaken until a uniform suspension is formed. The dissolution time of the vaccine should not exceed 1 min. The soluble drug should not contain precipitation or flakes. The place of inoculation - the outer surface of the middle third of the shoulder - is treated with alcohol or a mixture of alcohol and ether; use of other disinfectants is not allowed. After evaporation of alcohol and ether, without touching the skin, two drops of vaccine are applied at a distance of 30-40 mm from each other, the skin is stretched and a 6-incision (3 longitudinal and 3 transverse) 10 mm long with each scriber the distance between the notches is 3 mm.The serrations should not bleed, the blood should only act as a dewdrop.
The flat side of the scriber rubs the vaccine into the incisions for 30 seconds and allowed to dry for 5 minutes.
When revaccination, use a half dose, i.e. 1 drop of vaccine, through which 6 incisions are made.
Subcutaneous vaccination
The vaccine dose of the drug with this method is 25 times less than with the cutaneous vaccination; dilution of the vaccine is made at the rate of 12.5 ml of sodium chloride solution for injection 0.9% per dose of vaccine for cutaneous scarification (subcutaneous dose - 0.5 ml x 25 = 12.5 ml).
The preparation is dissolved in the same way as for the cutaneous vaccination, after which the resulting suspension is transferred to a sterile injector bottle into which the required volume of the solvent is introduced (for example, if the ampoule contains 7 doses of the vaccine, the contents should be suspended in 12.5 ml x 7, i.e. in 87.5 ml).
The vaccine is injected with an injector designed for subcutaneous administration (BI-3M or anti-infective protector PPI-2), according to the Instructions for the use of an injector in a volume of 0.5 ml.
Vaccinations are carried out in the area of the outer surface of the shoulder at the boundary between the upper and middle third.The injection site is treated in the same way as for the cutaneous inoculation.
Precautions for use.
It is strictly forbidden to inject the vaccine diluted for the cutaneous scarification application subcutaneously.
It is not possible to use a vaccine whose package integrity is damaged, with changed physical properties (foreign impurities, insoluble flakes), expired, if storage conditions are violated.
Opening of ampoules and the procedure for administering the drug are carried out with strict adherence to aseptic and antiseptic rules. A diluted vaccine, preserved with aseptic rules, can be used for 2 hours.
Given the possibility of developing an anaphylactic shock in certain highly sensitive individuals, the vaccinated should be under medical supervision for at least 30 minutes. Vaccination sites should be provided with anti-shock therapy.