The vaccine is given once by the skin method of scarification at a dose of 0.05 ml.
Revaccination is carried out at a dose of 0.05 ml not earlier than 1 year after the initial vaccination to persons whose serum lacks specific complement-binding antibodies.
In the ampoule with the vaccine, 0.5 ml of sodium chloride of the solvent for the preparation of medicinal forms for injection 0.9% is added immediately before inoculation. The vaccine should dissolve within 2 minutes to form a thick, homogeneous suspension of pink color with a grayish, yellowish or brownish hue.
The place of grafting (the outer surface of the middle third of the shoulder) is treated with 70% alcohol or ether solution (the use of other disinfectant solutions is not allowed). After their evaporation, 2 drops of diluted vaccine are applied at a distance of 30-40 mm through an eye dropper or syringe through a needle. Sterile scarifier, through each drop of vaccine applied to the skin, three cross-shaped incisions of 8-10 mm in length are made 3-4 mm apart so that the blood appears through small "dewdrops".The flat side of the scarifier is rubbed into the incision vaccine, after which the vaccine should dry for 5-10 minutes. The subsequent processing of the site is not carried out.
The dissolved vaccine is usable for 30 minutes when stored under a sterile tissue at room temperature and for 60 minutes when stored at a temperature of 2 to 10 ° C.
The introduction of the drug is recorded in the prescribed registration forms with an indication of the date of administration, dose, manufacturer, serial number, response to vaccination.