The sample is performed according to the prescription of the doctor to children, adolescents and adults by a specially trained nurse who has the admission to conduct intradermal tests. The drug is administered strictly intracutaneously.To carry out the test, use tuberculin syringes and thin short needles with an oblique cut. Before use it is necessary to check the date of their release and the expiration date.
The bottle can not be stored for more than 2 hours after opening. A syringe is collected 0.2 ml (two doses) of Diaskintest ® and the solution is discharged to a label of 0.1 ml in a sterile cotton swab.
The sample is carried out by the subject in the sitting position. After treating the skin area on the inner surface of the middle third of the forearm with 70% ethyl alcohol, 0.1 ml of Diaskintest® is injected into the upper layers of the stretched skin parallel to its surface. When setting the sample, as a rule, a papule is formed in the skin in the form of a "lemon crust" 7-10 mm in diameter, whitish in color.
Persons who have had a history of nonspecific allergy should be tested against desensitizing drugs within 7 days (5 days before and 2 days after the test).
Profitability Analysis
The result of the test is assessed by a doctor or a trained nurse after 72 hours from her setting, by measuring the transverse (relative to the forearm axis) transverse (in relation to the forearm axis) hypermia and infiltrate (papules), in mm. Hyperemia is only considered if there is no infiltrate.
The criteria for evaluating the response to the Diaskintest® sample are as follows:
| Reaction Category | Criteria for evaluation |
| Negative | Complete absence of infiltration and hyperemia or presence of a "knock-off reaction" up to 2 mm. |
| Doubtful | Presence of hyperemia without infiltration. |
| Positive | The presence of infiltrate (papules) of any size. |
| Slightly expressed | Infiltrate size is up to 5 mm. |
| Moderately expressed | The size of the infiltration is from 5 to 9 mm. |
| Expressive | The size of the infiltration is from 10 to 14 mm. |
| Hyperergic | The size of the infiltrate is 15 mm or more; vesiculonecal changes; and (or) lymphangitis, lymphadenitis, regardless of the size of the infiltrate. |
In contrast to the delayed-type hypersensitivity reaction, skin manifestations of nonspecific allergy (mainly hyperemia) to the drug are usually observed immediately after the sample is placed and usually disappear after 48-72 h. Diaskintest® does not cause delayed-type hypersensitivity reactions associated with BCG vaccination.
Persons with a dubious and positive reaction to Diaskintest® are subject to a comprehensive examination for tuberculosis.
Not all infected individuals develop delayed-type hypersensitivity for the Diaskintest® trial.There are factors that reduce this reaction, such as: HIV, active tuberculosis with severe immunopathological disorders caused by the severe course of the process; concomitant diseases accompanied by immunodeficiency.
In the registration documents for the registration of the results of the sample indicate: a) the name of the drug; b) manufacturer, serial number, expiry date; c) date of sampling; d) place of injection (left or right forearm); e) the result of the sample.