The method of blood collection is performed in accordance with the approved by the Ministry of Health of the Russian Federation on May 29, 1995, the "Instruction for the preparation and conservation of donor blood" (in the edition of the letter of the Ministry of Health of the Russian Federation dated 08.09.1999 N 2510/9770-99-32).
1. Before taking blood, the bottle with the solution is visually monitored, paying attention to its integrity, tightness, appearance of the solution, its transparency, and shelf life.
In the presence of cracks in the glass, violations of sealing, sealing, clouding of the solution or mechanical impurities (pile, fragments of cork or glass, etc.) - bottles with a solution can not be used.
2. Procurement of blood from a donor is performed using a sterile plastic system for taking a single-use blood.
3. On bottles with a preserving solution sealed with metal caps covering the rubber stopper, the metal plate is bent at the notch with a sterile tool, giving it a vertical position, exposing the central part of the rubber plug.
The part of the rubber plug thus opened is subsequently treated with a swab soaked in alcohol, then in a 5% alcohol solution of iodine, or a 0.5% solution of iodopyrone, or a 0.5% solution of iodonate.
4. Open the central part of the plug by piercing the airway needle to half its length, then the second needle connected to the blood collection system until it stops. It is recommended to insert the needles with the plane of the cut upwards at a slight angle to each other and at an angle of 65-70 ° to the plug.
5. Mounted bottles with vials are placed on a pedestal located below the level of the elbow of the donor.Bottles with a preservative solution should be at taking blood in an inclined position to prevent "foaming".
6. As the bottle is filled, the blood is periodically mixed with the preservative solution by gentle rocking and circular movements (manually or automatically).
7. If blood enters the bottle with droplets or ceases to flow, it should immediately be found and the cause of this is determined (change the position of the needle in the vein, replace the air filter, shift the tourniquet on the donor's hand, etc.).
When verifying the correct application of the tow or changing the air filter, a clamp should be applied to the blood collection system. If the blood supply stops due to a blockage of the airway needle with a piece of rubber stopper, immediately remove the clamp by placing a clamp on the donor tube and replace the impenetrable airway with a new one in order to avoid air embolism. When checking the patency of the system for taking blood, it is not allowed to violate its tightness.
8. After the end of the collection of blood, a clamp is placed on the system and the needle is transferred from the main vial into the vial-satellite, which, after removal of the clamp, is filled with blood,coming from the vein of the donor. The system is pinched, the needle is removed from the donor's vein and a sterile bandage is applied to the venipuncture site. After removing the clamp, the second vial is filled with blood remaining in the system. Blood from satellite vials is used to carry out control serological and biochemical analyzes.
9. After taking the blood from the donor, the needle with the air filter is removed from the bottle with the stored blood. The place of a puncture of a stopper by an antiseptic is processed, the leaf of a metal cap is established in initial (horizontal) position.
10. It is obligatory in the presence of the donor to verify the surname, name, patronymic of the donor on the credit card, and, separating the marks from it, stick them on a bottle of canned blood and vials.
11. The throat of the bottle is paraffinized by immersion in molten hot paraffin, covered with clean parchment paper (cellophane or polyethylene film) and dressed with a string of string or a chemist's rubber band. Instead of paraffin, the sealing of bottles with blood can be done with Unna paste.
12. A "passport" of blood is glued to the bottle, while the factory label is retained.
13. Packed and sealed glass bottles with blood and satellites attached to them in the shortest possible time after the workpiece are placed in special metal nets and transferred to storage in a refrigerator at a temperature of 2-6 ° C.